Characterization of a new family of proteins that interact with the C-terminal region of the chromatin-remodeling factor CHD-3.
The two human proteins Ki-1/57 and CGI-55 have highly similar amino acid sequences but their functions are unknown. We analyzed them by yeast two-hybrid screens and found that they interact with the C-terminal region of the human chromatin-remodeling factor CHD-3 (chromo-helicase-DNA-binding domain protein-3). The interaction of CGI-55 and CHD-3 could ... be confirmed in vitro and in vivo by co-immunoprecipitations from Sf9 insect cells. Mapping showed that CGI-55 interacts with CHD-3 via two regions at its N- and C-terminals. The CGI-55 and Ki-1/57 mRNAs show highest expression in muscle, colon and kidney. A CGI55-GFP fusion protein was localized in the cytoplasm, nucleus and perinuclear regions of HeLa cells. These data suggest the possibility that CGI-55 and Ki-1/57 might be involved in nuclear functions like the remodeling of chromatin.
Mesh Terms:
Adenosine Triphosphatases, Amino Acid Sequence, Animals, Antigens, CD30, Antigens, CD44, Binding Sites, Caenorhabditis elegans Proteins, Chromatin, DNA Helicases, Gene Expression, Hela Cells, Humans, Molecular Sequence Data, RNA, Messenger, RNA-Binding Proteins, Recombinant Proteins, Sequence Homology, Amino Acid, Tissue Distribution
Adenosine Triphosphatases, Amino Acid Sequence, Animals, Antigens, CD30, Antigens, CD44, Binding Sites, Caenorhabditis elegans Proteins, Chromatin, DNA Helicases, Gene Expression, Hela Cells, Humans, Molecular Sequence Data, RNA, Messenger, RNA-Binding Proteins, Recombinant Proteins, Sequence Homology, Amino Acid, Tissue Distribution
FEBS Lett.
Date: Jan. 02, 2003
PubMed ID: 12505151
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