Characterization of a new family of proteins that interact with the C-terminal region of the chromatin-remodeling factor CHD-3.

The two human proteins Ki-1/57 and CGI-55 have highly similar amino acid sequences but their functions are unknown. We analyzed them by yeast two-hybrid screens and found that they interact with the C-terminal region of the human chromatin-remodeling factor CHD-3 (chromo-helicase-DNA-binding domain protein-3). The interaction of CGI-55 and CHD-3 could ...
be confirmed in vitro and in vivo by co-immunoprecipitations from Sf9 insect cells. Mapping showed that CGI-55 interacts with CHD-3 via two regions at its N- and C-terminals. The CGI-55 and Ki-1/57 mRNAs show highest expression in muscle, colon and kidney. A CGI55-GFP fusion protein was localized in the cytoplasm, nucleus and perinuclear regions of HeLa cells. These data suggest the possibility that CGI-55 and Ki-1/57 might be involved in nuclear functions like the remodeling of chromatin.
Mesh Terms:
Adenosine Triphosphatases, Amino Acid Sequence, Animals, Antigens, CD30, Antigens, CD44, Binding Sites, Caenorhabditis elegans Proteins, Chromatin, DNA Helicases, Gene Expression, Hela Cells, Humans, Molecular Sequence Data, RNA, Messenger, RNA-Binding Proteins, Recombinant Proteins, Sequence Homology, Amino Acid, Tissue Distribution
FEBS Lett.
Date: Jan. 02, 2003
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