Phosphorylation of cold shock domain/Y-box proteins by ERK2 and GSK3beta and repression of the human VEGF promoter.

The hypoxia responsive region (HRR) of the VEGF promoter plays a key role in regulating VEGF expression. We found that the cold shock domain (Y-box) repressor proteins, dbpA and dbpB/YB-1, bind distinct strands of the human VEGF HRR. We find both dbpA and dbpB are phosphorylated by ERK2 and GSK3beta ...
in vitro, and the binding of dbpB to single-strand VEGF HRR DNA is regulated by this phosphorylation. These findings suggest the ERK/MAPK and PI3K pathways may regulate VEGF expression in part through regulating the action of these repressor proteins.
Mesh Terms:
Base Sequence, Cell Line, Tumor, DNA, Glycogen Synthase Kinase 3, Humans, Mitogen-Activated Protein Kinase 1, Molecular Sequence Data, Phosphorylation, Promoter Regions, Genetic, Vascular Endothelial Growth Factor A
FEBS Lett.
Date: Oct. 10, 2005
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