The envelope protein of severe acute respiratory syndrome coronavirus interacts with the non-structural protein 3 and is ubiquitinated.

To analyze the proteins interacting with the severe acute respiratory syndrome coronavirus (SARS-CoV) envelope (E) protein, a SARS-CoV was engineered including two tags associated to the E protein. Using this virus, complexes of SARS-CoV E and other proteins were purified using a tandem affinity purification system. Several viral and cell ...
proteins including spike, membrane, non-structural protein 3 (nsp3), dynein heavy chain, fatty acid synthase and transmembrane protein 43 bound E protein. In the present work, we focused on the binding of E protein to nsp3 in infected cells and cell-free systems. This interaction was mediated by the N-terminal acidic domain of nsp3. Moreover, nsp3 and E protein colocalized during the infection. It was shown that E protein was ubiquitinated in vitro and in cell culture, suggesting that the interaction between nsp3 and E protein may play a role in the E protein ubiquitination status and therefore on its turnover.
Mesh Terms:
Animals, Cell Line, Cercopithecus aethiops, Chromatography, Affinity, Humans, Protein Binding, Protein Interaction Domains and Motifs, Protein Interaction Mapping, Protein Processing, Post-Translational, RNA Replicase, SARS Virus, Ubiquitination, Viral Envelope Proteins, Viral Nonstructural Proteins
Virology
Date: Jul. 05, 2010
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