Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose).
Addition of poly(ADP-ribose) (PAR) is an important post-translational modification in higher eukaryotes. Several DNA repair and checkpoint proteins possess specific PAR-binding zinc-finger (PBZ) modules critical for function. Here, we present solution structures of the two PBZ modules of aprataxin and PNK-like factor (APLF), revealing a novel type of zinc finger. ... By combining in vivo PAR-binding data with NMR interaction data using PAR fragments, we propose a structural basis for PBZ-PAR recognition.
Mesh Terms:
Amino Acid Sequence, Humans, Models, Molecular, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Phosphoproteins, Poly Adenosine Diphosphate Ribose, Protein Binding, Protein Structure, Tertiary, Zinc Fingers
Amino Acid Sequence, Humans, Models, Molecular, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Phosphoproteins, Poly Adenosine Diphosphate Ribose, Protein Binding, Protein Structure, Tertiary, Zinc Fingers
Nat. Struct. Mol. Biol.
Date: Feb. 01, 2010
PubMed ID: 20098424
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