Human p300 protein is a coactivator for the transcription factor MyoD.

Human p300 protein is a cellular target of adenoviral E1A oncoprotein and a potential transcriptional coactivator. Both p300 and Rb family protein-binding regions of E1A are required for the repression of muscle gene expression, which is regulated by MyoD family transactivators. This implies that p300 is involved in MyoD-dependent transactivation. ...
We show that the repression of MyoD-mediated E box (MyoD consensus) reporter activity by E1A is correlated with its interaction with p300, indicating that p300 participates in MyoD-dependent transactivation. In addition, p300 is able to interact both in vivo and in vitro with MyoD through a portion at the carboxyl-terminal cysteine/histidine-rich domain and associates with the components of the basal transcriptional complex through its two separate transactivation domains at the amino and carboxyl termini. Consistent with its role as a coactivator, p300 potentiates MyoD-activated transcription.
Mesh Terms:
Cysteine, Enhancer Elements, Genetic, Histidine, Humans, Macromolecular Substances, MyoD Protein, Nuclear Proteins, Promoter Regions, Genetic, Protein Binding, Sequence Deletion, Structure-Activity Relationship, Trans-Activators, Transcription Factors, Transcription, Genetic, Transcriptional Activation
J. Biol. Chem.
Date: Apr. 12, 1996
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