Reduced MAP kinase phosphatase-1 degradation after p42/p44MAPK-dependent phosphorylation.
The mitogen-activated protein (MAP) kinase cascade is inactivated at the level of MAP kinase by members of the MAP kinase phosphatase (MKP) family, including MKP-1. MKP-1 was a labile protein in CCL39 hamster fibroblasts; its degradation was attenuated by inhibitors of the ubiquitin-directed proteasome complex. MKP-1 was a target in ... vivo and in vitro for p42(MAPK) or p44(MAPK), which phosphorylates MKP-1 on two carboxyl-terminal serine residues, Serine 359 and Serine 364. This phosphorylation did not modify MKP-1's intrinsic ability to dephosphorylate p44(MAPK) but led to stabilization of the protein. These results illustrate the importance of regulated protein degradation in the control of mitogenic signaling.
Mesh Terms:
Animals, Blood, Cell Cycle Proteins, Cell Division, Cell Line, Cricetinae, Culture Media, Cysteine Endopeptidases, Cysteine Proteinase Inhibitors, Dual Specificity Phosphatase 1, Estradiol, Humans, Immediate-Early Proteins, Leucine, Leupeptins, MAP Kinase Signaling System, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases, Multienzyme Complexes, Mutation, Nitrophenols, Organophosphorus Compounds, Phosphoprotein Phosphatases, Phosphorylation, Proteasome Endopeptidase Complex, Protein Phosphatase 1, Protein Tyrosine Phosphatases, Ubiquitins
Animals, Blood, Cell Cycle Proteins, Cell Division, Cell Line, Cricetinae, Culture Media, Cysteine Endopeptidases, Cysteine Proteinase Inhibitors, Dual Specificity Phosphatase 1, Estradiol, Humans, Immediate-Early Proteins, Leucine, Leupeptins, MAP Kinase Signaling System, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases, Multienzyme Complexes, Mutation, Nitrophenols, Organophosphorus Compounds, Phosphoprotein Phosphatases, Phosphorylation, Proteasome Endopeptidase Complex, Protein Phosphatase 1, Protein Tyrosine Phosphatases, Ubiquitins
Science
Date: Dec. 24, 1999
PubMed ID: 10617468
View in: Pubmed Google Scholar
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