Rod/Zw10 complex is required for PIASy-dependent centromeric SUMOylation.

SUMO conjugation of cellular proteins is essential for proper progression of mitosis. PIASy, a SUMO E3 ligase, is required for mitotic SUMOylation of chromosomal proteins, yet the regulatory mechanism behind the PIASy-dependent SUMOylation during mitosis has not been determined. Using a series of truncated PIASy proteins, we have found that ...
the N terminus of PIASy is not required for SUMO modification in vitro but is essential for mitotic SUMOylation in Xenopus egg extracts. We demonstrate that swapping the N terminus of PIASy protein with the corresponding region of other PIAS family members abolishes chromosomal binding and mitotic SUMOylation. We further show that the N-terminal domain of PIASy is sufficient for centromeric localization. We identified that the N-terminal domain of PIASy interacts with the Rod/Zw10 complex, and immunofluorescence further reveals that PIASy colocalizes with Rod/Zw10 in the centromeric region. We show that the Rod/Zw10 complex interacts with the first 47 residues of PIASy which were particularly important for mitotic SUMOylation. Finally, we show that depletion of Rod compromises the centromeric localization of PIASy and SUMO2/3 in mitosis. Together, we demonstrate a fundamental mechanism of PIASy to localize in the centromeric region of chromosome to execute centromeric SUMOylation during mitosis.
Mesh Terms:
Animals, Centromere, Chromosomal Proteins, Non-Histone, Humans, Kinetochores, Microtubule-Associated Proteins, Mitosis, Protein Binding, Protein Inhibitors of Activated STAT, Recombinant Fusion Proteins, Repressor Proteins, Small Ubiquitin-Related Modifier Proteins, Sumoylation, Xenopus Proteins, Xenopus laevis
J. Biol. Chem.
Date: Oct. 15, 2010
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