Identification of SUMOylated proteins in neuroblastoma cells after treatment with hydrogen peroxide or ascorbate.

The small ubiquitin-like modifier (SUMO) proteins have been implicated in the pathology of a number of diseases, including neurodegenerative diseases. The conjugation machinery for SUMOylation consists of a number of proteins which are redox sensitive. Here, under oxidative stress (100 μM hydrogen peroxide), antioxidant (100 μM ascorbate) or control conditions ...
169 proteins were identified by electrospray ionisation fourier transform ion cyclotron resonance mass spectrometry. The majority of these proteins (70%) were found to contain SUMOylation consensus sequences. From the remaining proteins a small number (12%) were found to contain possible SUMO interacting motifs. The proteins identified included DNA and RNA binding proteins, structural proteins and proteasomal proteins. Several of the proteins identified under oxidative stress conditions had previously been identified as SUMOylated proteins, thus validating the method presented.
Mesh Terms:
Amino Acid Sequence, Antioxidants, Ascorbic Acid, Cell Line, Tumor, Humans, Hydrogen Peroxide, Mass Spectrometry, Neuroblastoma, Oxidants, Oxidative Stress, Proteins, Sumoylation
BMB Rep
Date: Nov. 01, 2010
127533
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  • PTM Genes 168
Displaying 168 genes with ptm sites.
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Sumoylation (1)

ACAT1

acetyl-CoA acetyltransferase 1
Organism: Homo sapiens
Sumoylation (1)

ACOT7

acyl-CoA thioesterase 7
Organism: Homo sapiens
Sumoylation (1)

ACTB

actin, beta
Organism: Homo sapiens
Sumoylation (1)

ACTBL2

actin, beta-like 2
Organism: Homo sapiens
Sumoylation (1)

ACTC1

actin, alpha, cardiac muscle 1
Organism: Homo sapiens
Sumoylation (1)

ACTR1A

ARP1 actin-related protein 1 homolog A, centractin alpha (yeast)
Organism: Homo sapiens
Sumoylation (1)

ADSL

adenylosuccinate lyase
Organism: Homo sapiens
Sumoylation (1)

AHCY

adenosylhomocysteinase
Organism: Homo sapiens
Sumoylation (1)

AHSA1

AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
Organism: Homo sapiens
Sumoylation (1)

AIMP2

aminoacyl tRNA synthetase complex-interacting multifunctional protein 2
Organism: Homo sapiens
Sumoylation (1)

ANXA2

annexin A2
Organism: Homo sapiens
Sumoylation (1)

APEX1

APEX nuclease (multifunctional DNA repair enzyme) 1
Organism: Homo sapiens
Sumoylation (1)

API5

apoptosis inhibitor 5
Organism: Homo sapiens
Sumoylation (1)

ARFIP1

ADP-ribosylation factor interacting protein 1
Organism: Homo sapiens
Sumoylation (1)

ASAP2

ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Organism: Homo sapiens
Sumoylation (1)

ATP5A1

ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Organism: Homo sapiens
Sumoylation (1)

ATP5B

ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Organism: Homo sapiens
Sumoylation (1)

BIRC8

baculoviral IAP repeat containing 8
Organism: Homo sapiens
E3
Sumoylation (1)

BLVRA

biliverdin reductase A
Organism: Homo sapiens
Sumoylation (1)

BRIX1

BRX1, biogenesis of ribosomes, homolog (S. cerevisiae)
Organism: Homo sapiens
Sumoylation (1)

BUB3

BUB3 mitotic checkpoint protein
Organism: Homo sapiens
Sumoylation (1)

CCT2

chaperonin containing TCP1, subunit 2 (beta)
Organism: Homo sapiens
Sumoylation (1)

COQ6

coenzyme Q6 monooxygenase
Organism: Homo sapiens
Sumoylation (1)

CSNK2A1

casein kinase 2, alpha 1 polypeptide
Organism: Homo sapiens
TAUM
APO
BIOLOGICALRHYTHMS
CELLCYCLE
TRANSCRIPT
TRANSCRIPTREG
WNTSP
Sumoylation (1)

DARS

aspartyl-tRNA synthetase
Organism: Homo sapiens
Sumoylation (1)

DDX6

DEAD (Asp-Glu-Ala-Asp) box helicase 6
Organism: Homo sapiens
Sumoylation (1)

DDX39A

DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A
Organism: Homo sapiens
Sumoylation (1)

DDX39B

DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
Organism: Homo sapiens
Sumoylation (1)

DDX47

DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
Organism: Homo sapiens
Sumoylation (1)

DEK

DEK proto-oncogene
Organism: Homo sapiens
Sumoylation (1)

DNAJA1

DnaJ (Hsp40) homolog, subfamily A, member 1
Organism: Homo sapiens
TAUM
Sumoylation (1)

DNAJA4

DnaJ (Hsp40) homolog, subfamily A, member 4
Organism: Homo sapiens
Sumoylation (1)

DRG1

developmentally regulated GTP binding protein 1
Organism: Homo sapiens
Sumoylation (1)

EEF1A1

eukaryotic translation elongation factor 1 alpha 1
Organism: Homo sapiens
Sumoylation (1)

EEF1A2

eukaryotic translation elongation factor 1 alpha 2
Organism: Homo sapiens
Sumoylation (1)

EEF1G

eukaryotic translation elongation factor 1 gamma
Organism: Homo sapiens
Sumoylation (1)

EEF2

eukaryotic translation elongation factor 2
Organism: Homo sapiens
Sumoylation (1)

EIF2S3

eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
Organism: Homo sapiens
Sumoylation (1)

EIF4A1

eukaryotic translation initiation factor 4A1
Organism: Homo sapiens
Sumoylation (1)

EIF4A2

eukaryotic translation initiation factor 4A2
Organism: Homo sapiens
Sumoylation (1)

EIF4A3

eukaryotic translation initiation factor 4A3
Organism: Homo sapiens
Sumoylation (1)

ELAVL3

ELAV like neuron-specific RNA binding protein 3
Organism: Homo sapiens
Sumoylation (1)

ELAVL4

ELAV like neuron-specific RNA binding protein 4
Organism: Homo sapiens
Sumoylation (1)

ENO1

enolase 1, (alpha)
Organism: Homo sapiens
Sumoylation (1)

ENO2

enolase 2 (gamma, neuronal)
Organism: Homo sapiens
Sumoylation (1)

ENO3

enolase 3 (beta, muscle)
Organism: Homo sapiens
Sumoylation (1)

EPHX1

epoxide hydrolase 1, microsomal (xenobiotic)
Organism: Homo sapiens
Sumoylation (1)

FAM98B

family with sequence similarity 98, member B
Organism: Homo sapiens
Sumoylation (1)

FBL

fibrillarin
Organism: Homo sapiens
Sumoylation (1)

FBLL1

fibrillarin-like 1
Organism: Homo sapiens
Sumoylation (1)

FLOT1

flotillin 1
Organism: Homo sapiens
Sumoylation (1)

GAPDH

glyceraldehyde-3-phosphate dehydrogenase
Organism: Homo sapiens
Sumoylation (1)

GNA12

guanine nucleotide binding protein (G protein) alpha 12
Organism: Homo sapiens
Sumoylation (1)

GNAL

guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type
Organism: Homo sapiens
Sumoylation (1)

GNAS

GNAS complex locus
Organism: Homo sapiens
Sumoylation (1)

GNAT2

guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2
Organism: Homo sapiens
Sumoylation (1)

GOPC

golgi-associated PDZ and coiled-coil motif containing
Organism: Homo sapiens
Sumoylation (1)

H2AFY

H2A histone family, member Y
Organism: Homo sapiens
Sumoylation (1)

H2AFY2

H2A histone family, member Y2
Organism: Homo sapiens
Sumoylation (1)

H3F3C

H3 histone, family 3C
Organism: Homo sapiens
Sumoylation (1)

HARS

histidyl-tRNA synthetase
Organism: Homo sapiens
Sumoylation (1)

HDGF

hepatoma-derived growth factor
Organism: Homo sapiens
Sumoylation (1)

HERC2

HECT and RLD domain containing E3 ubiquitin protein ligase 2
Organism: Homo sapiens
E3
Sumoylation (1)

HIST1H1B

histone cluster 1, H1b
Organism: Homo sapiens
Sumoylation (1)

HIST1H1C

histone cluster 1, H1c
Organism: Homo sapiens
Sumoylation (1)

HIST1H1D

histone cluster 1, H1d
Organism: Homo sapiens
Sumoylation (1)

HIST1H1T

histone cluster 1, H1t
Organism: Homo sapiens
Sumoylation (1)

HIST1H2AB

histone cluster 1, H2ab
Organism: Homo sapiens
Sumoylation (1)

HIST1H2AE

histone cluster 1, H2ae
Organism: Homo sapiens
Sumoylation (1)

HIST1H2BB

histone cluster 1, H2bb
Organism: Homo sapiens
Sumoylation (1)

HIST1H4A

histone cluster 1, H4a
Organism: Homo sapiens
Sumoylation (1)

HIST1H4B

histone cluster 1, H4b
Organism: Homo sapiens
Sumoylation (1)

HIST1H4C

histone cluster 1, H4c
Organism: Homo sapiens
Sumoylation (1)

HIST1H4D

histone cluster 1, H4d
Organism: Homo sapiens
Sumoylation (1)

HIST1H4E

histone cluster 1, H4e
Organism: Homo sapiens
Sumoylation (1)

HIST1H4F

histone cluster 1, H4f
Organism: Homo sapiens
Sumoylation (1)

HIST1H4H

histone cluster 1, H4h
Organism: Homo sapiens
Sumoylation (1)

HIST1H4I

histone cluster 1, H4i
Organism: Homo sapiens
Sumoylation (1)

HIST1H4J

histone cluster 1, H4j
Organism: Homo sapiens
Sumoylation (1)

HIST1H4K

histone cluster 1, H4k
Organism: Homo sapiens
Sumoylation (1)

HIST1H4L

histone cluster 1, H4l
Organism: Homo sapiens
Sumoylation (1)

HIST2H2BC

histone cluster 2, H2bc (pseudogene)
Organism: Homo sapiens
Sumoylation (1)

HIST2H4A

histone cluster 2, H4a
Organism: Homo sapiens
Sumoylation (1)

HIST2H4B

histone cluster 2, H4b
Organism: Homo sapiens
Sumoylation (1)

HIST4H4

histone cluster 4, H4
Organism: Homo sapiens
Sumoylation (1)

HNRNPA0

heterogeneous nuclear ribonucleoprotein A0
Organism: Homo sapiens
Sumoylation (1)

HNRNPA1

heterogeneous nuclear ribonucleoprotein A1
Organism: Homo sapiens
Sumoylation (1)

HNRNPA2B1

heterogeneous nuclear ribonucleoprotein A2/B1
Organism: Homo sapiens
Sumoylation (1)

HNRNPA3

heterogeneous nuclear ribonucleoprotein A3
Organism: Homo sapiens
Sumoylation (1)

HNRNPAB

heterogeneous nuclear ribonucleoprotein A/B
Organism: Homo sapiens
Sumoylation (1)

HNRNPC

heterogeneous nuclear ribonucleoprotein C (C1/C2)
Organism: Homo sapiens
Sumoylation (1)

HNRNPCL1

heterogeneous nuclear ribonucleoprotein C-like 1
Organism: Homo sapiens
Sumoylation (1)

HNRNPD

heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
Organism: Homo sapiens
BIOLOGICALRHYTHMS
TRANSCRIPT
TRANSCRIPTREG
Sumoylation (1)

HNRNPDL

heterogeneous nuclear ribonucleoprotein D-like
Organism: Homo sapiens
Sumoylation (1)

HNRNPF

heterogeneous nuclear ribonucleoprotein F
Organism: Homo sapiens
Sumoylation (1)

HNRNPH1

heterogeneous nuclear ribonucleoprotein H1 (H)
Organism: Homo sapiens
Sumoylation (1)

HNRNPH2

heterogeneous nuclear ribonucleoprotein H2 (H')
Organism: Homo sapiens
Sumoylation (1)

HNRNPH3

heterogeneous nuclear ribonucleoprotein H3 (2H9)
Organism: Homo sapiens
Sumoylation (1)

HSP90AB1

heat shock protein 90kDa alpha (cytosolic), class B member 1
Organism: Homo sapiens
TAUM
AMYL
Sumoylation (1)

HSP90B1

heat shock protein 90kDa beta (Grp94), member 1
Organism: Homo sapiens
Sumoylation (1)

ICK

intestinal cell (MAK-like) kinase
Organism: Homo sapiens
Sumoylation (1)

ILF2

interleukin enhancer binding factor 2
Organism: Homo sapiens
Sumoylation (1)

INADL

InaD-like (Drosophila)
Organism: Homo sapiens
Sumoylation (1)

MLX

MLX, MAX dimerization protein
Organism: Homo sapiens
Sumoylation (1)

MTBP

MDM2 binding protein
Organism: Homo sapiens
Sumoylation (1)

NACA

nascent polypeptide-associated complex alpha subunit
Organism: Homo sapiens
UBP
Sumoylation (1)

NACA2

nascent polypeptide-associated complex alpha subunit 2
Organism: Homo sapiens
UBP
Sumoylation (1)

NAP1L1

nucleosome assembly protein 1-like 1
Organism: Homo sapiens
Sumoylation (1)

NCOA5

nuclear receptor coactivator 5
Organism: Homo sapiens
Sumoylation (1)

NEK10

NIMA-related kinase 10
Organism: Homo sapiens
Sumoylation (1)

NFIB

nuclear factor I/B
Organism: Homo sapiens
Sumoylation (1)

NPM1

nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Organism: Homo sapiens
Sumoylation (1)

NPTX1

neuronal pentraxin I
Organism: Homo sapiens
Sumoylation (1)

OTUD6B

OTU domain containing 6B
Organism: Homo sapiens
DUB
Sumoylation (1)

PA2G4

proliferation-associated 2G4, 38kDa
Organism: Homo sapiens
Sumoylation (1)

PAICS

phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
Organism: Homo sapiens
Sumoylation (1)

PCBP1

poly(rC) binding protein 1
Organism: Homo sapiens
Sumoylation (1)

PCBP2

poly(rC) binding protein 2
Organism: Homo sapiens
Sumoylation (1)

PDIA6

protein disulfide isomerase family A, member 6
Organism: Homo sapiens
Sumoylation (1)

PGK1

phosphoglycerate kinase 1
Organism: Homo sapiens
Sumoylation (1)

POLE

polymerase (DNA directed), epsilon, catalytic subunit
Organism: Homo sapiens
Sumoylation (1)

POLG2

polymerase (DNA directed), gamma 2, accessory subunit
Organism: Homo sapiens
Sumoylation (1)

POTEE

POTE ankyrin domain family, member E
Organism: Homo sapiens
Sumoylation (1)

PPP1CC

protein phosphatase 1, catalytic subunit, gamma isozyme
Organism: Homo sapiens
TAUM
Sumoylation (1)

PPP2R2A

protein phosphatase 2, regulatory subunit B, alpha
Organism: Homo sapiens
TAUM
Sumoylation (1)

PRSS50

protease, serine, 50
Organism: Homo sapiens
Sumoylation (1)

PSIP1

PC4 and SFRS1 interacting protein 1
Organism: Homo sapiens
TRANSCRIPTION
Sumoylation (1)

PSMC3

proteasome (prosome, macropain) 26S subunit, ATPase, 3
Organism: Homo sapiens
PROT
Sumoylation (1)

PSMC4

proteasome (prosome, macropain) 26S subunit, ATPase, 4
Organism: Homo sapiens
PROT
Sumoylation (1)

PSMD11

proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
Organism: Homo sapiens
PROT
TAUM
NOTAVAILABLE
Sumoylation (1)

PSMD12

proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
Organism: Homo sapiens
PROT
Sumoylation (1)

RALY

RALY heterogeneous nuclear ribonucleoprotein
Organism: Homo sapiens
Sumoylation (1)

RALYL

RALY RNA binding protein-like
Organism: Homo sapiens
Sumoylation (1)

RBMX

RNA binding motif protein, X-linked
Organism: Homo sapiens
Sumoylation (1)

RBMXL2

RNA binding motif protein, X-linked-like 2
Organism: Homo sapiens
Sumoylation (1)

RCC1

regulator of chromosome condensation 1
Organism: Homo sapiens
Sumoylation (1)

RPF2

ribosome production factor 2 homolog (S. cerevisiae)
Organism: Homo sapiens
Sumoylation (1)

RPL3

ribosomal protein L3
Organism: Homo sapiens
Sumoylation (1)

RPL4

ribosomal protein L4
Organism: Homo sapiens
Sumoylation (1)

RPL5

ribosomal protein L5
Organism: Homo sapiens
OTHER
Sumoylation (1)

RPL6

ribosomal protein L6
Organism: Homo sapiens
Sumoylation (1)

RPLP0P6

ribosomal protein, large, P0 pseudogene 6
Organism: Homo sapiens
Sumoylation (1)

RPSA

ribosomal protein SA
Organism: Homo sapiens
Sumoylation (1)

RUVBL1

RuvB-like AAA ATPase 1
Organism: Homo sapiens
Sumoylation (1)

RUVBL2

RuvB-like AAA ATPase 2
Organism: Homo sapiens
Sumoylation (1)

SEPT6

septin 6
Organism: Homo sapiens
Sumoylation (1)

SMC1B

structural maintenance of chromosomes 1B
Organism: Homo sapiens
Sumoylation (1)

TFAP2A

transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Organism: Homo sapiens
Sumoylation (1)

TFAP2B

transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
Organism: Homo sapiens
Sumoylation (1)

TOP2B

topoisomerase (DNA) II beta 180kDa
Organism: Homo sapiens
Sumoylation (1)

TRA2A

transformer 2 alpha homolog (Drosophila)
Organism: Homo sapiens
Sumoylation (1)

TRIM4

tripartite motif containing 4
Organism: Homo sapiens
E3
Sumoylation (1)

TUBA1A

tubulin, alpha 1a
Organism: Homo sapiens
Sumoylation (1)

TUBA1B

tubulin, alpha 1b
Organism: Homo sapiens
Sumoylation (1)

TUBA3E

tubulin, alpha 3e
Organism: Homo sapiens
Sumoylation (1)

TUBA4B

tubulin, alpha 4b (pseudogene)
Organism: Homo sapiens
Sumoylation (1)

TUBB

tubulin, beta class I
Organism: Homo sapiens
Sumoylation (1)

TUBB1

tubulin, beta 1 class VI
Organism: Homo sapiens
Sumoylation (1)

TUBB2B

tubulin, beta 2B class IIb
Organism: Homo sapiens
Sumoylation (1)

TUBB3

tubulin, beta 3 class III
Organism: Homo sapiens
Sumoylation (1)

TUBB4A

tubulin, beta 4A class IVa
Organism: Homo sapiens
Sumoylation (1)

TUBB4B

tubulin, beta 4B class IVb
Organism: Homo sapiens
Sumoylation (1)

TUBB6

tubulin, beta 6 class V
Organism: Homo sapiens
Sumoylation (1)

TUBB8

tubulin, beta 8 class VIII
Organism: Homo sapiens
Sumoylation (1)

TUFM

Tu translation elongation factor, mitochondrial
Organism: Homo sapiens
Sumoylation (1)

UBB

ubiquitin B
Organism: Homo sapiens
UB
UPS
FACOMPLEX
TAUM
Sumoylation (1)

UBR4

ubiquitin protein ligase E3 component n-recognin 4
Organism: Homo sapiens
E3
Sumoylation (1)

UROC1

urocanate hydratase 1
Organism: Homo sapiens