Molecular cloning and characterization of a novel cbl-family gene, cbl-c.
We have cloned a novel gene, cbl-c, of mammalian cbl-family. The cbl-c gene is predicted to encode a protein of 52 kDa that has a phosphotyrosine-binding domain, a RING finger and a proline-rich region. Cbl-c shows 50% homology to the amino-terminal sequences of Cbl and Cbl-b, but a sequence corresponding ... to the carboxy-terminal half of Cbl and Cbl-b is largely missing in Cbl-c. The expression of cbl-c mRNA is distinct from that of cbl and cbl-b mRNAs, being high in the colon and small intestine, but undetectable in brain and lymphoid tissues. The cbl-c gene is mapped in 19q13.2-13.3. Finally, the 52 kDa Cbl-c protein binds to the EGF receptor and Fyn tyrosine kinase. We conclude that Cbl-c is a novel Cbl-family adaptor protein that would regulate intracellular signaling mediated by various tyrosine kinases.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Chromosome Mapping, Chromosomes, Human, Pair 19, Cloning, Molecular, DNA, Complementary, Female, Gene Expression, Humans, In Situ Hybridization, Fluorescence, Male, Molecular Sequence Data, Protein Binding, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-cbl, Proto-Oncogene Proteins c-fyn, RNA, Messenger, Receptor, Epidermal Growth Factor, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Tissue Distribution, Ubiquitin-Protein Ligases
Amino Acid Sequence, Base Sequence, Chromosome Mapping, Chromosomes, Human, Pair 19, Cloning, Molecular, DNA, Complementary, Female, Gene Expression, Humans, In Situ Hybridization, Fluorescence, Male, Molecular Sequence Data, Protein Binding, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-cbl, Proto-Oncogene Proteins c-fyn, RNA, Messenger, Receptor, Epidermal Growth Factor, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Tissue Distribution, Ubiquitin-Protein Ligases
Gene
Date: Oct. 18, 1999
PubMed ID: 10571044
View in: Pubmed Google Scholar
Download Curated Data For This Publication
12814
Switch View:
- Interactions 3