Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress.
The substrates of SUMO4, a novel member for the SUMO gene family, were characterized in HEK293 cells cultured under serum starvation by proteomic analysis. We identified 90 SUMO4 substrates including anti-stress proteins such as antioxidant enzymes and molecular chaperones or co-chaperones. The substrates also include proteins involved in the regulation ... of DNA repair and synthesis, RNA processing, protein degradation, and glucose metabolism. Several SUMO4-associated transcription factors were characterized by Western blot analyses. AP-1 was selected for in vitro conjugation assays to confirm SUMO4 sumoylation of these transcription factors. Further functional analyses of the transcription factors suggested that SUMO4 sumoylation represses AP-1 and AP-2alpha transcriptional activity, but enhances GR DNA binding capacity. These results demonstrate that SUMO4 sumoylation may play an important role in the regulation of intracellular stress.
Mesh Terms:
Cell Line, Culture Media, Serum-Free, Gene Expression Regulation, Humans, Protein Binding, Proteomics, Proto-Oncogene Proteins c-fos, Small Ubiquitin-Related Modifier Proteins, Substrate Specificity, Transcription Factors, Transcription, Genetic
Cell Line, Culture Media, Serum-Free, Gene Expression Regulation, Humans, Protein Binding, Proteomics, Proto-Oncogene Proteins c-fos, Small Ubiquitin-Related Modifier Proteins, Substrate Specificity, Transcription Factors, Transcription, Genetic
Biochem. Biophys. Res. Commun.
Date: Dec. 02, 2005
PubMed ID: 16236267
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