Interaction between Saccharomyces cerevisiae Glutaredoxin 5 (Grx5) and SPT10 and the in vivo Function.
Glutaredoxin5 (Grx5) is a monothiol member of the Grx family that comprises two dithiol and three monothiol members. Using a yeast two-hybrid system, we isolated a Grx5-binding protein, SPT10, which has been previously suggested to act as a global transcriptional regulator of specific histone genes. We find that among the ... five members of Grx family and two members of Trx family (Trx1 and Trx2), Grx5 alone interacts with SPT10 via an intermolecular disulfide linkage between the 60Cys of Grx5 and 385Cys of SPT10. To evaluate the physiological function of the Grx5/SPT10 interaction, we investigated the phenotypes of three null mutant strains (Grx5Δ, SPT10Δ, and Grx5ΔSPT10Δ). Taken together, these results show that all of these phenotypes are probably a consequence of disruption of the interaction between Grx5 and SPT10. In our present study, we suggest the interaction between Grx5 and SPT10 via intermolecular disulfide linkage and propose a model for a role of Grx5 in the regulation of protein expressions under the control of SPT10.
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Date: Feb. 08, 2012
PubMed ID: 22326886
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