Gene-specific RNA polymerase II phosphorylation and the CTD code.

Phosphorylation of the RNA polymerase (Pol) II C-terminal domain (CTD) repeats (1-YSPTSPS-7) is coupled to transcription and may act as a 'code' that controls mRNA synthesis and processing. To examine the code in budding yeast, we mapped genome-wide CTD Ser2, Ser5 and Ser7 phosphorylations and the CTD-associated termination factors Nrd1 ...
and Pcf11. Phospho-CTD dynamics are not scaled to gene length and are gene-specific, with highest Ser5 and Ser7 phosphorylation at the 5' ends of well-expressed genes with nucleosome-occupied promoters. The CTD kinases Kin28 and Ctk1 markedly affect Pol II distribution in a gene-specific way. The code is therefore written differently on different genes, probably under the control of promoters. Ser7 phosphorylation is enriched on introns and at sites of Nrd1 accumulation, suggesting links to splicing and Nrd1 recruitment. Nrd1 and Pcf11 frequently colocalize, suggesting functional overlap. Unexpectedly, Pcf11 is enriched at centromeres and Pol III-transcribed genes.
Mesh Terms:
Chromatin Immunoprecipitation, Cyclin-Dependent Kinases, DNA, Fungal, Genes, Fungal, Genome-Wide Association Study, Introns, Macromolecular Substances, Models, Genetic, Phosphorylation, Phosphoserine, Promoter Regions, Genetic, Protein Kinases, Protein Processing, Post-Translational, RNA Polymerase II, RNA, Fungal, RNA, Messenger, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors
Nat. Struct. Mol. Biol.
Date: Oct. 01, 2010
129355
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