CKS proteins protect mitochondrial genome integrity by interacting with mitochondrial single-stranded DNA-binding protein.

Eukaryotic Chromatin Dynamics Group, Medical Research Council Clinical Sciences Centre, Imperial College Hammersmith Campus, Du Cane Road, London, UK.
Cyclin-dependent kinase subunit (CKS) proteins interact with cyclin-dependent kinases (CDKs) with high affinity. Mammalian CKS1 and CKS2 bind CDK1 and CDK2 and partake in the control of cell cycle progression. We identified CKS-interacting proteins by affinity purification followed by mass spectrometry in the human lymphocytic cell line Ramos. Apart from known interactors, such as CDKs, we identified a novel CDK-dependent interaction between CKS proteins and the mitochondrial single-stranded DNA-binding protein (mtSSB). mtSSB bound both CKS1 and CKS2 and underwent CDK-dependent phosphorylation. mtSSB is known to participate in replication of mitochondrial DNA. We demonstrated that mitochondrial morphology and DNA integrity were compromised in cells depleted of both CKS proteins or that had inhibited CDK activity. These features are consistent with the hypothesis of CKS-dependent regulation of mtSSB function and support a direct role of cell cycle proteins in controlling mitochondrial DNA replication.
Mesh Terms:
Animals, CDC2 Protein Kinase, Carrier Proteins, Cell Cycle Proteins, Cell Line, Cell Line, Tumor, Cyclin-Dependent Kinases, DNA, Mitochondrial, DNA, Single-Stranded, DNA-Binding Proteins, Electrophoresis, Gel, Two-Dimensional, Genome, Mitochondrial, Humans, Immunoblotting, Mass Spectrometry, Microscopy, Fluorescence, Mitochondria, Mitochondrial Proteins, Mutation, Phosphorylation, Protein Binding, Protein Kinases, RNA Interference, Recombinant Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Mol. Cell Proteomics Jan. 01, 2010; 9(1);145-52 [PUBMED:19786724]
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