Darby MM, Serebreni L, Pan X, Boeke JD, Corden JL

The Saccharomyces cerevisiae Nrd1-Nab3 pathway directs termination and processing of short RNA polymerase II transcripts. Despite the potential for Nrd1-Nab3 to affect transcription of both coding and non-coding RNAs, little is known about how the Nrd1-Nab3 pathway interacts with other pathways in the cell. Here we present the results of ...

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a high-throughput synthetic lethality screen for genes that interact with NRD1 and show roles for Nrd1 in regulation of mitochondrial abundance and cell size. We also provide genetic evidence of interaction between the Nrd1-Nab3 and Ras/PKA pathways. Whereas the Ras pathway promotes transcription of genes involved in growth and glycolysis, the Nrd1-Nab3 pathway appears to have a novel role in the rapid suppression of some genes when cells are shifted to poor growth conditions. We report the identification of new mRNA targets of the Nrd1-Nab3 pathway that are rapidly repressed in response to glucose depletion. Glucose depletion also leads to dephosphorylation of Nrd1 and the formation of novel nuclear speckles that contain Nrd1 and Nab3. Taken together, these results indicate a role for Nrd1-Nab3 in regulating cellular response to nutrient availability.

Date: Mar. 19, 2012

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