ETL, a novel seven-transmembrane receptor that is developmentally regulated in the heart. ETL is a member of the secretin family and belongs to the epidermal growth factor-seven-transmembrane subfamily.
Using differential display of rat fetal and postnatal cardiomyocytes, we have identified a novel seven-transmembrane receptor, ETL. The cDNA-predicted amino acid sequence of ETL indicated that it encodes a 738-aa protein composed of a large extracellular domain with epidermal growth factor (EGF)-like repeats, a seven-transmembrane domain, and a short cytoplasmic ... tail. ETL belongs to the secretin family of G-protein-coupled peptide hormone receptors and the EGF-TM7 subfamily of receptors. The latter are characterized by a variable number of extracellular EGF and cell surface domains and conserved seven transmembrane-spanning regions. ETL mRNA expression is up-regulated in the adult rat and human heart. In situ hybridization analyses revealed expression in rat cardiomyocytes and abundant expression in vascular and bronchiolar smooth muscle cells. In COS-7 cells transfected with Myc-tagged rat ETL, rat ETL exists as a stable dimer and undergoes endoproteolytic cleavage of the extracellular domain. The proteolytic activity can be abolished by a specific mutation, T455A, in this domain. In transfected mammalian cells, ETL is associated with cell membranes and is also observed in cytoplasmic vesicles. ETL is the first seven-transmembrane receptor containing EGF-like repeats that is developmentally regulated in the heart.
Mesh Terms:
Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, COS Cells, Chromosome Mapping, Chromosomes, Human, Pair 1, DNA Primers, Dimerization, Epidermal Growth Factor, Gene Expression Regulation, Developmental, Heart, Humans, Molecular Sequence Data, Myocardium, RNA, Messenger, Rats, Receptors, Cell Surface, Receptors, G-Protein-Coupled, Secretin, Sequence Homology, Amino Acid, Up-Regulation
Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, COS Cells, Chromosome Mapping, Chromosomes, Human, Pair 1, DNA Primers, Dimerization, Epidermal Growth Factor, Gene Expression Regulation, Developmental, Heart, Humans, Molecular Sequence Data, Myocardium, RNA, Messenger, Rats, Receptors, Cell Surface, Receptors, G-Protein-Coupled, Secretin, Sequence Homology, Amino Acid, Up-Regulation
J. Biol. Chem.
Date: Feb. 09, 2001
PubMed ID: 11050079
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