Yeast Swd2 is essential due to antagonism between the Set1 histone methyltransferase complex and the Associated with Pta1 (APT) termination factor.

The Set1 complex (also known as Complex associated with Set1 or COMPASS) methylates histone H3 on lysine 4, with different levels of methylation affecting transcription by recruiting various factors to distinct regions of active genes. Neither Set1 nor its associated proteins are essential for viability with the notable exception of ...
Swd2, a WD repeat protein that is also a subunit of the essential transcription termination factor APT (Associated with Pta1). Cells lacking Set1 lose COMPASS recruitment but show increased promoter crosslinking of TFIIE large subunit and the serine 5 phosphorylated form of the Rpb1 carboxy-terminal domain. While Swd2 is normally required for bringing APT to genes, deletion of SET1 restores both viability and APT recruitment to a strain lacking Swd2. We propose a model in which Swd2 is required for APT to overcome antagonism by COMPASS.
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Date: Mar. 19, 2012
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