[Roles of G-protein beta and gamma subunits in the interaction of G beta gamma with adenylyl cyclases II].

To explore the individual roles of G protein beta and gamma subunit in the interactions with effectors.We investigated the interactions of G beta 1 and G beta 1 gamma 2 with adenylyl cyclase II(AC II) using the yeast two-hybrid and three-hybrid systems.When assayed for the abilities to activate the reporter ...
gene, the interactions among AD-beta 1, gamma 2 and BD-AC IIQ in the three-hybrid system were more potent than the interactions between AD-beta 1 and BD-AC II Q in the two-hybrid system. The expressions of BD-AC IIQ and AD-beta 1 in transformants coexpressed AD-beta 1 and BD-AC IIQ, and transformants coexpressed AD-beta 1, gamma 2 and BD-AC IIQ were respectively detected. The comparisons between the reporter activity and the expression levels of BD-AC IIQ and AD-beta 1 in the yeast cells show there was no correlations, i.e. The difference in the reporter activity was not a reflection of differential expression level of the hybrid proteins.All these results suggest that G protein beta 1 subunit is sufficient to maintain the basic interaction between G beta 1 gamma 2 and AC IIQ, and gamma 2 subunit plays an important role in the high affinity interaction of G beta 1 gamma 2 with AC IIQ.
Mesh Terms:
Adenylate Cyclase, GTP-Binding Protein beta Subunits, GTP-Binding Protein gamma Subunits, Two-Hybrid System Techniques, Yeasts
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Date: Apr. 01, 2001
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