Regulation of LIM homeodomain activity in vivo: a tetramer of dLDB and apterous confers activity and capacity for regulation by dLMO.

Dorsal-ventral axis formation in the Drosophila wing depends on the activity of the LIM homeodomain transcription factor Apterous and its cofactor, dLDB/Chip. We present evidence that Apterous activity depends on the formation of a LIM homeodomain dimer bridged by a dimer of cofactor. We show that Apterous activity levels are ...
regulated in vivo by dLMO, an antagonist of homodimer formation. Making use of a constitutively active form of Apterous and dominant-negative forms of Apterous and dLDB/Chip, we show that the normal function of dLMO is to downregulate Apterous activity and that the dLMO mutant phenotype is due to excess Apterous activity. These findings may point to a general mechanism for regulation of LIM homeodomain protein activity.
Mesh Terms:
Animals, Body Patterning, Cloning, Molecular, Dimerization, Drosophila, Drosophila Proteins, Homeodomain Proteins, Insect Proteins, Macromolecular Substances, Nuclear Proteins, Polymerase Chain Reaction, Recombinant Proteins, Transcription Factors, Wing
Mol. Cell
Date: Aug. 01, 1999
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