Regulation of LIM homeodomain activity in vivo: a tetramer of dLDB and apterous confers activity and capacity for regulation by dLMO.
Dorsal-ventral axis formation in the Drosophila wing depends on the activity of the LIM homeodomain transcription factor Apterous and its cofactor, dLDB/Chip. We present evidence that Apterous activity depends on the formation of a LIM homeodomain dimer bridged by a dimer of cofactor. We show that Apterous activity levels are ... regulated in vivo by dLMO, an antagonist of homodimer formation. Making use of a constitutively active form of Apterous and dominant-negative forms of Apterous and dLDB/Chip, we show that the normal function of dLMO is to downregulate Apterous activity and that the dLMO mutant phenotype is due to excess Apterous activity. These findings may point to a general mechanism for regulation of LIM homeodomain protein activity.
Mesh Terms:
Animals, Body Patterning, Cloning, Molecular, Dimerization, Drosophila, Drosophila Proteins, Homeodomain Proteins, Insect Proteins, Macromolecular Substances, Nuclear Proteins, Polymerase Chain Reaction, Recombinant Proteins, Transcription Factors, Wing
Animals, Body Patterning, Cloning, Molecular, Dimerization, Drosophila, Drosophila Proteins, Homeodomain Proteins, Insect Proteins, Macromolecular Substances, Nuclear Proteins, Polymerase Chain Reaction, Recombinant Proteins, Transcription Factors, Wing
Mol. Cell
Date: Aug. 01, 1999
PubMed ID: 10488342
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