The relative expression amounts of apterous and its co-factor dLdb/Chip are critical for dorso-ventral compartmentalization in the Drosophila wing.
Dorso-ventral axis formation in the Drosophila wing requires the localized accumulation of the Apterous LIM/homeodomain protein (Ap) in dorsal cells. Here we report that dLdb/Chip encodes a LIM-binding cofactor that controls Ap activity. Both lack and excess of dLdb/Chip function cause the same phenotype as apterous (ap) lack of function; ... i.e. dorsal to ventral transformations, generation of new wing margins, and wing outgrowths. These results indicate that the normal function of Ap in dorso-ventral compartmentalization requires the correct amount of the DLDB/CHIP co-factor, and suggest that the Ap and DLDB/CHIP proteins form a multimeric functional complex. In support of this model, we show that the dLdb/Chip excess-of-function phenotypes can be rescued by ap overexpression.
Mesh Terms:
Animals, Animals, Genetically Modified, Body Patterning, Drosophila, Drosophila Proteins, Gene Expression, Guinea Pigs, Homeodomain Proteins, Insect Proteins, Mice, Mosaicism, Mutagenesis, Nuclear Proteins, Phenotype, Transcription Factors, Wing
Animals, Animals, Genetically Modified, Body Patterning, Drosophila, Drosophila Proteins, Gene Expression, Guinea Pigs, Homeodomain Proteins, Insect Proteins, Mice, Mosaicism, Mutagenesis, Nuclear Proteins, Phenotype, Transcription Factors, Wing
EMBO J.
Date: Dec. 01, 1998
PubMed ID: 9843490
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