Chip and apterous physically interact to form a functional complex during Drosophila development.
LIM homeodomain (LIM-HD) proteins play key roles in a variety of developmental processes throughout the animal kingdom. Here we show that the LIM-binding protein Chip acts as a cofactor for the Drosophila LIM-HD family member Apterous (Ap) in wing development. We define the domains of Chip required for LIM-HD binding ... and for homodimerization and show that mutant proteins deleted for these domains act in a dominant-negative fashion to disrupt Ap function. Our results support a model for multimeric complexes containing Chip and Ap in transcriptional regulation. This model is confirmed by the activity of a chimeric fusion between Chip and Ap that reconstitutes the complex and rescues the ap mutant phenotype.
Mesh Terms:
Animals, Animals, Genetically Modified, Dimerization, Drosophila, Drosophila Proteins, Gene Expression Regulation, Developmental, Genes, Reporter, Homeodomain Proteins, Insect Proteins, Nuclear Proteins, Recombinant Proteins, Transcription Factors, Wing
Animals, Animals, Genetically Modified, Dimerization, Drosophila, Drosophila Proteins, Gene Expression Regulation, Developmental, Genes, Reporter, Homeodomain Proteins, Insect Proteins, Nuclear Proteins, Recombinant Proteins, Transcription Factors, Wing
Mol. Cell
Date: Aug. 01, 1999
PubMed ID: 10488341
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