Chip and apterous physically interact to form a functional complex during Drosophila development.

LIM homeodomain (LIM-HD) proteins play key roles in a variety of developmental processes throughout the animal kingdom. Here we show that the LIM-binding protein Chip acts as a cofactor for the Drosophila LIM-HD family member Apterous (Ap) in wing development. We define the domains of Chip required for LIM-HD binding ...
and for homodimerization and show that mutant proteins deleted for these domains act in a dominant-negative fashion to disrupt Ap function. Our results support a model for multimeric complexes containing Chip and Ap in transcriptional regulation. This model is confirmed by the activity of a chimeric fusion between Chip and Ap that reconstitutes the complex and rescues the ap mutant phenotype.
Mesh Terms:
Animals, Animals, Genetically Modified, Dimerization, Drosophila, Drosophila Proteins, Gene Expression Regulation, Developmental, Genes, Reporter, Homeodomain Proteins, Insect Proteins, Nuclear Proteins, Recombinant Proteins, Transcription Factors, Wing
Mol. Cell
Date: Aug. 01, 1999
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