Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation.
General transcription factor TFIIH, previously described as a 10-subunit complex, is essential for transcription and DNA repair. An eleventh subunit now identified, termed Tfb6, exhibits 45% sequence similarity to human nuclear mRNA export factor 5. Tfb6 dissociates from TFIIH as a heterodimer with the Ssl2 subunit, a DNA helicase that ... drives promoter melting for the initiation of transcription. Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes.
Mesh Terms:
Chromatography, Liquid, DNA Helicases, Gene Deletion, Gene Expression Regulation, Fungal, Humans, Mass Spectrometry, Phenotype, Phosphorylation, Protein Binding, Protein Subunits, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, Transcription Factor TFIIH, Transcription Factors, Transcription, Genetic, Ultraviolet Rays
Chromatography, Liquid, DNA Helicases, Gene Deletion, Gene Expression Regulation, Fungal, Humans, Mass Spectrometry, Phenotype, Phosphorylation, Protein Binding, Protein Subunits, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, Transcription Factor TFIIH, Transcription Factors, Transcription, Genetic, Ultraviolet Rays
Proc. Natl. Acad. Sci. U.S.A.
Date: Mar. 27, 2012
PubMed ID: 22411836
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