Molecular Interactions of GBF1 with HY5 and HYH During Light-mediated Seedling Development in Arabidopsis thaliana.

Arabidopsis bZIP transcription factor, GBF1, acts as a differential regulator of cryptochrome-mediated blue light signaling. Whereas the bZIP proteins, Elongated Hypocotyl 5 (HY5) and HY5 Homologue (HYH), are degraded by COP1-mediated proteasomal pathways, GBF1 is degraded by a proteasomal pathway independent of COP1. In this study, we have investigated the ...
functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development. The genetic studies using double and triple mutants reveal that GBF1 largely acts antagonistically with HY5 and HYH in Arabidopsis seedling development. Further, GBF1 and HY5 play more important roles than HYH in blue light-mediated photomorphogenic growth. This study reveals that GBF1 is able to form G-box binding heterodimer with HY5, however not with HYH. The in vitro and in vivo studies demonstrate that GBF1 colocalizes with HY5 or HYH in the nucleus, and physically interacts with both the proteins. The protein-protein interaction studies further reveal that the bZIP domain of GBF1 is essential and sufficient for the interaction with HY5 or HYH. Taken together, these data demonstrate the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development.
Unknown
Date: Jun. 12, 2012
Download Curated Data For This Publication
132087
Switch View:
  • Interactions 7