Sos7, an essential component of the conserved S. pombe Ndc80-MIND-Spc7 complex, identifies a new family of fungal kinetochore proteins.

Chromosome segregation is powered by the kinetochore, a large macromolecular structure assembled on centromeric chromatin. Attachment of sister chromatids to microtubules is mediated by the highly conserved tripartite KMN (acronym for KNL-1-Mis12-Ndc80) kinetochore network. In the fission yeast S. pombe the equivalent complex is called NMS (Ndc80-MIND-Spc7). Here we show ...
that not all components of the NMS complex had been identified previously. A 10(th) NMS component exists, the essential Sos7 protein which is a genetic and physical interaction partner of Spc7. The analysis of sos7 kinetochore-null mutant yeast strains demonstrated that Sos7 is central to NMS function. In particular, Sos7 is required for kinetochore-targeting of Spc7 as well as components of the MIND complex. sos7 mutant strains show severe chromosome mis-segregation phenotypes and have compromised microtubule-kinetochore interactions.Sos7 is the founding member of a functionally conserved fungal kinetochore family not present in the point centromere carrying Saccharomycotina clusters suggesting that the new Sos7 family might be a signature motif of fungi with regional centromeres.
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Date: Jun. 18, 2012
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