Kim JY, Kim KB, Eom GH, Choe NW, Kee HJ, Son HJ, Oh ST, Kim DW, Pak JH, Baek HJ, Kook H, Hahn Y, Kook H, Chakravarti D, Seo SB

Histone lysine methylation and demethylation are considered critical steps in transcriptional regulation. In this report, we performed ChIP-chip analysis to examine the genome-wide occupancy of H3K9-me2 during all-trans retinoid acid (ATRA)-induced differentiation of HL-60 promyelocytic leukemia cells. Using this approach, we found that KDM3B, which contains a JmjC domain, was ...

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down-regulated during differentiation through the recruitment of a corepressor complex. Furthermore, KDM3B displayed histone H3K9-me1/2 demethylase activity and induced leukemogenic oncogene lmo2 expression via a synergistic interaction with CBP. Here, we found that KDM3B repressed leukemia cell differentiation and was up-regulated in blood cells from ALL type leukemia patients. The combined results of this study provide evidence that the H3K9-me1/2 demethylase KDM3B might play a role in leukemogenesis via activation of lmo2 through interdependent actions with the HAT complex containing CBP.

Date: May. 21, 2012

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