Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA.
BRCA2 is a tumor suppressor that functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on single-stranded DNA (ssDNA) to perform homology search and DNA strand invasion. We report the purification of full-length human BRCA2 and show that ... it binds to ~6 RAD51 molecules and promotes RAD51 binding to ssDNA coated by replication protein A (RPA), in a manner that is stimulated by DSS1.
Mesh Terms:
Apoptosis Regulatory Proteins, BRCA2 Protein, Chromatography, Affinity, DNA Repair, DNA, Circular, DNA, Single-Stranded, Humans, Models, Biological, Proteasome Endopeptidase Complex, Protein Binding, Protein Interaction Mapping, Rad51 Recombinase, Recombinant Fusion Proteins, Recombination, Genetic, Replication Protein A
Apoptosis Regulatory Proteins, BRCA2 Protein, Chromatography, Affinity, DNA Repair, DNA, Circular, DNA, Single-Stranded, Humans, Models, Biological, Proteasome Endopeptidase Complex, Protein Binding, Protein Interaction Mapping, Rad51 Recombinase, Recombinant Fusion Proteins, Recombination, Genetic, Replication Protein A
Nat. Struct. Mol. Biol.
Date: Oct. 01, 2010
PubMed ID: 20729859
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