Involvement of suppressor of cytokine signalling-1-mediated degradation of MyD88-adaptor-like protein in the suppression of Toll-like receptor 2-mediated signalling by the murine C-type lectin SIGNR1-mediated signalling.
Dendritic cells recognize pathogens through pattern recognition receptors such as Toll-like receptors and phagocytose and digest them by phagocytic receptors for antigen presentation. This study was designed to clarify the cross-talk between recognition and phagocytosis of microbes in dendritic cells. The murine dendritic cell line XS106 cells were stimulated with ... the murine C-type lectin SIGNR1 ligand lipoarabinomannan and the Toll-like receptor 2 ligand FSL-1. The co-stimulation significantly suppressed FSL-1-mediated activation of NF-κB as well as production of TNF-α, IL-6 and IL-12p40 in a dose-dependent manner. The suppression was significantly but not completely recovered by knock-down of SIGNR1. SIGNR1 was associated with Toll-like receptor 2 in XS106 cells. The co-stimulation upregulated the expression of suppressor of cytokine signalling-1 in XS106 cells, the knock-down of which almost completely recovered the suppression of the FSL-1-mediated cytokine production by lipoarabinomannan. In addition, it was found that the MyD88-adaptor-like protein in XS106 cells was degraded by co-stimulation with FSL-1 and lipoarabinomannan in the absence, but not the presence, of the proteasome inhibitor MG132 and the degradation was inhibited by knock-down of suppressor of cytokine signalling-1. This study suggests that Toll-like receptor 2-mediated signalling is negatively regulated by SIGNR1-mediated signalling in dendritic cells, possibly through suppressor of cytokine signalling-1-mediated degradation of the MyD88-adaptor-like protein.
Mesh Terms:
Animals, Cell Adhesion Molecules, Cell Line, Dendritic Cells, Diglycerides, HEK293 Cells, Humans, Interleukin-12 Subunit p40, Interleukin-6, Lectins, C-Type, Leupeptins, Lipopolysaccharides, Mice, Myeloid Differentiation Factor 88, NF-kappa B, Oligopeptides, Phagocytosis, Receptors, Cell Surface, Signal Transduction, Suppressor of Cytokine Signaling Proteins, Toll-Like Receptor 2, Tumor Necrosis Factor-alpha
Animals, Cell Adhesion Molecules, Cell Line, Dendritic Cells, Diglycerides, HEK293 Cells, Humans, Interleukin-12 Subunit p40, Interleukin-6, Lectins, C-Type, Leupeptins, Lipopolysaccharides, Mice, Myeloid Differentiation Factor 88, NF-kappa B, Oligopeptides, Phagocytosis, Receptors, Cell Surface, Signal Transduction, Suppressor of Cytokine Signaling Proteins, Toll-Like Receptor 2, Tumor Necrosis Factor-alpha
Cell. Microbiol.
Date: Jan. 01, 2012
PubMed ID: 21899704
View in: Pubmed Google Scholar
Download Curated Data For This Publication
132936
Switch View:
- Interactions 3
- PTM Genes 1