Rat alpha-synuclein interacts with Tat binding protein 1, a component of the 26S proteasomal complex.
The alpha-synuclein gene, which encodes a brain presynaptic nerve terminal protein of unknown function, is linked to familial early-onset Parkinson's disease (PD). The finding that alpha-synuclein forms the major fibrillary component of Lewy bodies in brains of PD patients suggests that the two point mutations in alpha-synuclein (Ala(53)Thr, Ala(30)Pro) may ... promote the aggregation of alpha-synuclein into filaments. To address the role of alpha-synuclein in neurodegenerative diseases, we performed a yeast two-hybrid screen of a rat adult brain cDNA library using rat alpha-synuclein 2 (alphaSYN2). Here we report that alphaSYN2 interacts specifically with Tat binding protein 1, a subunit of the 700-kDa proteasome activator (PA700), the regulatory complex of the 26S proteasome and of the modulator complex, which enhances PA700 activation of the proteasome.
Mesh Terms:
Animals, Brain, Cell Line, Clone Cells, DNA-Binding Proteins, Dopamine, Humans, Kidney, Lewy Bodies, Macromolecular Substances, Nerve Tissue Proteins, Neurons, Parkinsonian Disorders, Peptide Hydrolases, Precipitin Tests, Presynaptic Terminals, Proteasome Endopeptidase Complex, Proteins, Rats, Saccharomyces, Synucleins, Transfection, Two-Hybrid System Techniques, Tyrosine 3-Monooxygenase, alpha-Synuclein
Animals, Brain, Cell Line, Clone Cells, DNA-Binding Proteins, Dopamine, Humans, Kidney, Lewy Bodies, Macromolecular Substances, Nerve Tissue Proteins, Neurons, Parkinsonian Disorders, Peptide Hydrolases, Precipitin Tests, Presynaptic Terminals, Proteasome Endopeptidase Complex, Proteins, Rats, Saccharomyces, Synucleins, Transfection, Two-Hybrid System Techniques, Tyrosine 3-Monooxygenase, alpha-Synuclein
J. Neurochem.
Date: Nov. 01, 2000
PubMed ID: 11032911
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