Corcoles-Saez I, Ballester-Tomas L, de la Torre-Ruiz Sr MA, Prieto JA, Randez-Gil F

Unlike other stresses, the physiological significance and molecular mechanisms involved in the yeast cold response are largely unknown. Here, we show that the cell wall integrity (CWI) pathway plays an important role in the growth of S. cerevisiae at low temperatures. Cells lacking the Wsc1p membrane sensor or the MAPKs ...

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Bck1p, Mkk1p/Mkk2p or Slt2p exhibited cold sensitivity. However, there was no evidence of either a cold-provoked perturbation of the cell wall or a differential cold expression program mediated by Slt2p. Our results suggest that Slt2p is activated by different inputs in response to nutrient signals and mediates growth control through TORC1-Sch9p and PKA at low temperatures. We found that absence of TOR1 causes cold sensitivity, whereas a ras2Δ mutant shows increased cold growth. Lack of Sch9p alleviates the phenotype of slt2Δ and bck1Δ mutant cells, as well as attenuation of PKA activity by over-expression of BCY1. Interestingly, swi4Δ mutant cells display cold sensitivity, but the phenotype is neither mediated by the Slt2p-regulated induction of Swi4p-responsive promoters nor influenced by osmotic stabilisation. Hence, cold signalling through the CWI pathway has distinct features and might imply still unknown effectors and targets.

Date: Jun. 29, 2012

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