Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis.
Autophagy and apoptosis are two evolutionarily conserved processes that regulate cell fate in response to cytotoxic stress. However, the functional relationship between these two processes remains far from clear. Here, we demonstrate an autophagy-dependent mechanism of caspase-8 activation and initiation of the apoptotic cascade in response to SKI-I, a pan-sphingosine ... kinase inhibitor, and bortezomib, a proteasome inhibitor. Autophagy is induced concomitantly with caspase-8 activation, which is responsible for initiation of the caspase cascade and the mitochondrial amplification loop that is required for full execution of apoptosis. Inhibition of autophagosome formation by depletion of Atg5 or Atg3 results in a marked suppression of caspase-8 activation and apoptosis. Although caspase-8 self-association depends on p62/SQSTM1, its self-processing requires the autophagosomal membrane. Caspase-8 forms a complex with Atg5 and colocalizes with LC3 and p62. Moreover, FADD, an adaptor protein for caspase-8 activation, associates with Atg5 on Atg16L- and LC3-positive autophagosomal membranes and loss of FADD suppresses cell death. Taken together, these results indicate that the autophagosomal membrane serves as a platform for an intracellular death-inducing signaling complex (iDISC) that recruits self-associated caspase-8 to initiate the caspase-8/-3 cascade.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Animals, Apoptosis, Autophagy, Caspase 3, Caspase 8, Cell Membrane, Cell Survival, Cells, Cultured, Death Domain Receptor Signaling Adaptor Proteins, Enzyme Activation, Fas-Associated Death Domain Protein, Gene Knockout Techniques, Heat-Shock Proteins, Humans, Hydrazines, Leukemia, Myeloid, Acute, Lysosome-Associated Membrane Glycoproteins, Mice, Microtubule-Associated Proteins, Mitochondria, Phosphotransferases (Alcohol Group Acceptor), Protein Binding, Protein Multimerization, Protein Transport, Pyrazoles, Tumor Cells, Cultured, Ubiquitin-Conjugating Enzymes
Adaptor Proteins, Signal Transducing, Animals, Apoptosis, Autophagy, Caspase 3, Caspase 8, Cell Membrane, Cell Survival, Cells, Cultured, Death Domain Receptor Signaling Adaptor Proteins, Enzyme Activation, Fas-Associated Death Domain Protein, Gene Knockout Techniques, Heat-Shock Proteins, Humans, Hydrazines, Leukemia, Myeloid, Acute, Lysosome-Associated Membrane Glycoproteins, Mice, Microtubule-Associated Proteins, Mitochondria, Phosphotransferases (Alcohol Group Acceptor), Protein Binding, Protein Multimerization, Protein Transport, Pyrazoles, Tumor Cells, Cultured, Ubiquitin-Conjugating Enzymes
J. Biol. Chem.
Date: Apr. 06, 2012
PubMed ID: 22362782
View in: Pubmed Google Scholar
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