Abl-SH3 binding protein 2, 3BP2, interacts with CIN85 and HIP-55.
The adapter 3BP2 is involved in leukocyte signaling downstream Src/Syk-kinases coupled immunoreceptors. Here, we show that 3BP2 directly interacts with the endocytic scaffold protein CIN85 and the actin-binding protein HIP-55. 3BP2 co-localized with CIN85 and HIP-55 in T cell rafts and at the T cell/APC synapse, an active zone of ... receptors and proteins recycling. A binding region of CIN85 SH3 domains on 3BP2 was mapped to a PVPTPR motif in the first proline-rich region of 3BP2, whereas the C-terminal SH3 domain of HIP-55 bound a more distal proline-rich domain of 3BP2. Together, our data suggest an unexpected role of 3BP2 in endocytic and cytoskeletal regulation through its interaction with CIN85 and HIP-55.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Motifs, Amino Acid Sequence, Binding Sites, Cell Line, Cytoskeleton, Endocytosis, Humans, Jurkat Cells, Microfilament Proteins, Models, Biological, Proline, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins, T-Lymphocytes, Two-Hybrid System Techniques, src Homology Domains
Adaptor Proteins, Signal Transducing, Amino Acid Motifs, Amino Acid Sequence, Binding Sites, Cell Line, Cytoskeleton, Endocytosis, Humans, Jurkat Cells, Microfilament Proteins, Models, Biological, Proline, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins, T-Lymphocytes, Two-Hybrid System Techniques, src Homology Domains
FEBS Lett.
Date: Mar. 06, 2007
PubMed ID: 17306257
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