Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain.
The ESCRT-I and ESCRT-II complexes help sort ubiquitinated proteins into vesicles that accumulate within multivesicular bodies (MVBs). Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the beta-sandwich. The ... structure suggests how human ESCRT-II can couple recognition of ubiquitinated cargoes and endosomal phospholipids during MVB protein sorting.
Mesh Terms:
Amino Acid Motifs, Binding Sites, Carrier Proteins, Crystallography, X-Ray, Endosomal Sorting Complexes Required for Transport, Endosomes, Humans, Models, Molecular, Molecular Sequence Data, Protein Folding, Protein Structure, Tertiary, Protein Transport, Recombinant Fusion Proteins, Transport Vesicles, Ubiquitin, Vesicular Transport Proteins
Amino Acid Motifs, Binding Sites, Carrier Proteins, Crystallography, X-Ray, Endosomal Sorting Complexes Required for Transport, Endosomes, Humans, Models, Molecular, Molecular Sequence Data, Protein Folding, Protein Structure, Tertiary, Protein Transport, Recombinant Fusion Proteins, Transport Vesicles, Ubiquitin, Vesicular Transport Proteins
Nat. Struct. Mol. Biol.
Date: Nov. 01, 2006
PubMed ID: 17057716
View in: Pubmed Google Scholar
Download Curated Data For This Publication
134700
Switch View:
- Interactions 2