Initial crystallographic study of human PCNA in complex with a peptide containing the noncanonical PIP-box sequence of human DNA polymerase iota.

Human DNA polymerase iota (Poliota) is one of the Y-family DNA polymerases involved in translesion synthesis (TLS), which allows continued replication at damaged DNA templates. Poliota has a noncanonical PCNA-interacting protein box (PIP-box) within an internal region of the protein. Poliota activity is stimulated by PCNA binding through the noncanonical ...
PIP-box. To clarify the interaction of PCNA with the noncanonical PIP-box of Poliota, PCNA and a Poliota peptide carrying the noncanonical PIP-box complex have been cocrystallized. The crystal belongs to space group C2, with unit-cell parameters a = 167.1, b = 68.7, c = 90.0 A, beta = 95.1 degrees . Structural analysis by molecular replacement is in progress.
Mesh Terms:
DNA Damage, DNA Replication, DNA-Directed DNA Polymerase, Humans, Peptide Fragments, Peptides, Proliferating Cell Nuclear Antigen, Recombinant Proteins, X-Ray Diffraction
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
Date: Oct. 01, 2008
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