Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery.

During cytokinesis, as dividing animal cells pull apart into two daughter cells, the final stage, termed abscission, requires breakage of the midbody, a thin membranous stalk connecting the daughter cells. This membrane fission event topologically resembles the budding of viruses, such as HIV-1, from infected cells. We found that two ...
proteins involved in HIV-1 budding-tumor susceptibility gene 101 (Tsg101), a subunit of the endosomal sorting complex required for transport I (ESCRT-I), and Alix, an ESCRT-associated protein-were recruited to the midbody during cytokinesis by interaction with centrosome protein 55 (Cep55), a centrosome and midbody protein essential for abscission. Tsg101, Alix, and possibly other components of ESCRT-I were required for the completion of cytokinesis. Thus, HIV-1 budding and cytokinesis use a similar subset of cellular components to carry out topologically similar membrane fission events.
Mesh Terms:
Adenosine Triphosphatases, Amino Acid Motifs, Calcium-Binding Proteins, Carrier Proteins, Cell Cycle Proteins, Cytokinesis, DNA-Binding Proteins, Endosomal Sorting Complexes Required for Transport, Endosomes, HIV-1, HeLa Cells, Humans, Microtubules, Nuclear Proteins, Protein Binding, Protein Structure, Tertiary, R-SNARE Proteins, RNA Interference, Recombinant Fusion Proteins, Syntaxin 1, Transcription Factors, Transfection, Vesicular Transport Proteins
Science
Date: Jun. 29, 2007
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