Physical and functional interactions between Daxx and TSG101.

Daxx has been reported to mediate the Fas/JNK-dependent signals in the cytoplasm. However, several evidences have suggested that Daxx is located mainly in the nucleus and functions as a transcriptional regulator. Recently, we identified DMAP1, a TSG101-interacting protein as a Daxx binding partner by yeast two-hybrid screening. TSG101 has been ...
shown to act as transcriptional co-repressor of nuclear hormone receptors. Here we examined whether TSG101also interacts with Daxx directly. The association of Daxx and TSG101 was confirmed using co-expressed tagged proteins. The interaction regions in both proteins were also mapped, and the cellular localization of the interaction was examined. TSG101 formed a complex with Daxx through its coiled-coil domain and co-localized in the nucleus. Furthermore, TSG101 enhanced Daxx-mediated repression of glucocorticoid receptor transcriptional activity. These results provide the novel molecular interactions between Daxx and TSG101, which establish an efficient repressive transcription complex in the nucleus.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Animals, COS Cells, Carrier Proteins, Cell Line, Cell Nucleus, Cytoplasm, DNA-Binding Proteins, Dose-Response Relationship, Drug, Endosomal Sorting Complexes Required for Transport, Fluorescent Antibody Technique, Indirect, Humans, Immunoblotting, Intracellular Signaling Peptides and Proteins, Luciferases, Nuclear Proteins, Precipitin Tests, Protein Binding, Protein Structure, Tertiary, Receptors, Glucocorticoid, Transcription Factors, Transcription, Genetic, Transfection, Two-Hybrid System Techniques
Biochem. Biophys. Res. Commun.
Date: Apr. 09, 2004
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