The p53 target protein Wig-1 binds hnRNP A2/B1 and RNA Helicase A via RNA.
The p53-induced Wig-1 gene encodes a double stranded RNA-binding zinc finger protein. We generated Saos-2 osteosarcoma cells expressing tetracycline-inducible Flag-tagged human Wig-1. Induction of Wig-1 expression by doxycycline inhibited cell growth in a long-term assay but did not cause any changes in cell cycle distribution nor increased fraction of apoptotic ... cells. Using co-immunoprecipitation and mass spectrometry, we identified two Wig-1-binding proteins, hnRNP A2/B1 and RNA Helicase A, both of which are involved in RNA processing. The binding was dependent on the presence of RNA. Our results establish a link between the p53 tumor suppressor and RNA processing via hnRNPA2/B1 and RNA Helicase A.
Mesh Terms:
Carrier Proteins, DEAD-box RNA Helicases, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, Immunoprecipitation, Neoplasm Proteins, Nuclear Proteins, Point Mutation, RNA, RNA Processing, Post-Transcriptional, Tetracycline, Tumor Suppressor Protein p53, Zinc Fingers
Carrier Proteins, DEAD-box RNA Helicases, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, Immunoprecipitation, Neoplasm Proteins, Nuclear Proteins, Point Mutation, RNA, RNA Processing, Post-Transcriptional, Tetracycline, Tumor Suppressor Protein p53, Zinc Fingers
FEBS Lett.
Date: Jun. 25, 2008
PubMed ID: 18519039
View in: Pubmed Google Scholar
Download Curated Data For This Publication
135460
Switch View:
- Interactions 4