Analysis of the interaction between GGA1 GAT domain and Rabaptin-5.
GGAs are a family of adaptor proteins involved in vesicular transport. As an effector of the small GTPase Arf, GGA interacts using its GAT domain with the GTP-bound form of Arf. The GAT domain is also found to interact with ubiquitin and rabaptin-5. Rabaptin-5 is, in turn, an effector of ... another small GTPase, Rab5, which regulates early endosome fusion. The interaction between GGAs and rabaptin-5 is likely to take place in a pathway between the trans-Golgi network and early endosomes. This chapter describes in vitro biochemical characterization of the interaction between the GGA1 GAT domain and rabaptin-5. Combining with the complex crystal structure, we reveal that the binding mode is helix bundle-to-helix bundle in nature.
Mesh Terms:
ADP-Ribosylation Factors, Adaptor Proteins, Vesicular Transport, Cell Line, DNA, Complementary, Humans, Protein Binding, Vesicular Transport Proteins
ADP-Ribosylation Factors, Adaptor Proteins, Vesicular Transport, Cell Line, DNA, Complementary, Humans, Protein Binding, Vesicular Transport Proteins
Meth. Enzymol.
Date: Feb. 14, 2006
PubMed ID: 16473621
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