The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression.
Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development.We provide new evidence showing that overexpression of heterodimeric AP-1 comprised of c-jun and c-fos (AP-1(c-Jun/c-Fos)) induces the expression of BMP-antagonizing organizer ... genes (noggin, chordin and goosecoid) that were normally expressed by high dose of activin. AP-1(c-Jun/c-Fos) enhanced the promoter activities of organizer genes but reduced that of PV.1, a BMP4-response gene. A loss of function study clearly demonstrated that AP-1(c-Jun/c-Fos) is required for the activin-induced organizer and neural gene expression. Moreover, physical interaction of AP-1(c-Jun/c-Fos) and Smad3 cooperatively enhanced the transcriptional activity of goosecoid via direct binding on this promoter. Interestingly, Smad3 mutants at c-Jun binding site failed in regulation of organizer genes, indicating that these physical interactions are specifically necessary for the expression of organizer genes.AP-1(c-Jun/c-Fos) plays a specific role in organizer gene expression in downstream of activin signal during early Xenopus embryogenesis.
Mesh Terms:
Activins, Animals, Blotting, Western, Bone Morphogenetic Protein 4, Carrier Proteins, Chromatin Immunoprecipitation, Embryo, Nonmammalian, Gene Expression Regulation, Glycoproteins, Goosecoid Protein, Immunoprecipitation, In Situ Hybridization, Intercellular Signaling Peptides and Proteins, Luciferases, Organizers, Embryonic, Promoter Regions, Genetic, Protein Binding, Protein Multimerization, Proto-Oncogene Proteins c-fos, Proto-Oncogene Proteins c-jun, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Smad3 Protein, T-Box Domain Proteins, Transcription Factor AP-1, Transcriptional Activation, Xenopus Proteins, Xenopus laevis
Activins, Animals, Blotting, Western, Bone Morphogenetic Protein 4, Carrier Proteins, Chromatin Immunoprecipitation, Embryo, Nonmammalian, Gene Expression Regulation, Glycoproteins, Goosecoid Protein, Immunoprecipitation, In Situ Hybridization, Intercellular Signaling Peptides and Proteins, Luciferases, Organizers, Embryonic, Promoter Regions, Genetic, Protein Binding, Protein Multimerization, Proto-Oncogene Proteins c-fos, Proto-Oncogene Proteins c-jun, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Smad3 Protein, T-Box Domain Proteins, Transcription Factor AP-1, Transcriptional Activation, Xenopus Proteins, Xenopus laevis
PLoS ONE
Date: Aug. 11, 2011
PubMed ID: 21829441
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