Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation.
GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface ... impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing.
Mesh Terms:
Amino Acid Substitution, Crystallography, X-Ray, Humans, MicroRNAs, Models, Molecular, Mutagenesis, Site-Directed, Poly(A)-Binding Protein I, Protein Binding, Protein Interaction Mapping, Protein Structure, Quaternary, RNA, Messenger, RNA-Binding Proteins
Amino Acid Substitution, Crystallography, X-Ray, Humans, MicroRNAs, Models, Molecular, Mutagenesis, Site-Directed, Poly(A)-Binding Protein I, Protein Binding, Protein Interaction Mapping, Protein Structure, Quaternary, RNA, Messenger, RNA-Binding Proteins
Nat. Struct. Mol. Biol.
Date: Feb. 01, 2010
PubMed ID: 20098421
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