SOCS36E, a novel Drosophila SOCS protein, suppresses JAK/STAT and EGF-R signalling in the imaginal wing disc.

We have cloned a novel SOCS gene from Drosophila, socs36E, which is most homologous to the mammalian socs-5 gene. Socs36E is expressed zygotically, predominantly during embryogenesis, in a highly dynamic pattern. In vivo expression of SOCS36E in transgenic flies results in several adult phenotypes. Engrailed-GAL4 directed expression causes loss of ...
the wing anterior cross vein, humeral outgrowths, absence of halteres and eye pigmentation defects. Expression of SOCS36E under apterous-GAL4 control resulted in outstretched wings. Full penetrance of these phenotypes required the presence of the SH2 and SOCS-box domains of SOCS36E. The observed phenotypes were consistent with defects in JAK/STAT or EGF-R signalling and were exacerbated in flies heterozygous for either the d-jak (hopscotch), d-stat (stat92E) or d-egf-r (der) genes. Conversely, inactivating one copy of the d-cbl gene, a negative regulator of the d-EGF-R, partially rescued the wing phenotypes. These genetic interactions imply that SOCS36E can suppress activities of the JAK/STAT and EGF-R signalling pathways in the wing disc and suggest that SOCS36E interacts with multiple pathways in vivo.
Mesh Terms:
Amino Acid Sequence, Animals, Animals, Genetically Modified, Cell Line, Cloning, Molecular, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Humans, Janus Kinases, Mice, Molecular Sequence Data, Protein Kinases, Protein-Tyrosine Kinases, RNA, Messenger, Receptor, Epidermal Growth Factor, Receptors, Invertebrate Peptide, Repressor Proteins, STAT Transcription Factors, Sequence Alignment, Signal Transduction, Suppressor of Cytokine Signaling Proteins, Trans-Activators, Transcription Factors, Wing
Oncogene
Date: Jul. 18, 2002
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