Exploring the role of p97 and its UBX-domain cofactors through identification of their interacting proteins.
The elucidation of protein-protein interaction networks can provide preliminary insights into the function of uncharacterized proteins based on the interactions they establish in the cell. Here, we describe a protein immunoprecipitation protocol that can be used in combination with mass spectrometry analysis to identify the p97 interactome as well as ... specific subgroups of proteins interacting with its UBX-domain adaptors. This approach aims to dissect the role played by individual UBX cofactors within the complex array of cellular functions performed by p97.
Mesh Terms:
Adenosine Triphosphatases, Cell Cycle Proteins, Cell Line, HEK293 Cells, Humans, Immunoprecipitation, Mass Spectrometry, Protein Binding, Protein Interaction Domains and Motifs, Protein Interaction Maps, Ubiquitin, Ubiquitinated Proteins
Adenosine Triphosphatases, Cell Cycle Proteins, Cell Line, HEK293 Cells, Humans, Immunoprecipitation, Mass Spectrometry, Protein Binding, Protein Interaction Domains and Motifs, Protein Interaction Maps, Ubiquitin, Ubiquitinated Proteins
Methods Mol. Biol.
Date: Feb. 22, 2012
PubMed ID: 22350894
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