Oncoprotein MDM2 is a ubiquitin ligase E3 for tumor suppressor p53.
The tumor suppressor p53 is degraded by the ubiquitin-proteasome system. p53 was polyubiquitinated in the presence of E1, UbcH5 as E2 and MDM2 oncoprotein. A ubiquitin molecule bound MDM2 through sulfhydroxy bond which is characteristic of ubiquitin ligase (E3)-ubiquitin binding. The cysteine residue in the carboxyl terminus of MDM2 was ... essential for the activity. These data suggest that the MDM2 protein, which is induced by p53, functions as a ubiquitin ligase, E3, in human papillomavirus-uninfected cells which do not have E6 protein.
Mesh Terms:
Amino Acid Sequence, Cell Extracts, HeLa Cells, Humans, Ligases, Molecular Sequence Data, Nuclear Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-mdm2, Sequence Analysis, Tumor Suppressor Protein p53, Ubiquitin-Conjugating Enzymes, Ubiquitins
Amino Acid Sequence, Cell Extracts, HeLa Cells, Humans, Ligases, Molecular Sequence Data, Nuclear Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-mdm2, Sequence Analysis, Tumor Suppressor Protein p53, Ubiquitin-Conjugating Enzymes, Ubiquitins
FEBS Lett.
Date: Dec. 22, 1997
PubMed ID: 9450543
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