Human Fas-associated factor 1, interacting with ubiquitinated proteins and valosin-containing protein, is involved in the ubiquitin-proteasome pathway.

Human Fas-associated factor 1 (hFAF1) is a novel protein having multiubiquitin-related domains. We investigated the cellular functions of hFAF1 and found that valosin-containing protein (VCP), the multiubiquitin chain-targeting factor in the degradation of the ubiquitin-proteasome pathway, is a binding partner of hFAF1. hFAF1 is associated with the ubiquitinated proteins via ...
the newly identified N-terminal UBA domain and with VCP via the C-terminal UBX domain. The overexpression of hFAF1 and a truncated UBA domain inhibited the degradation of ubiquitinated proteins and increased cell death. These results suggest that hFAF1 binding to ubiquitinated protein and VCP is involved in the ubiquitin-proteasome pathway. We hypothesize that hFAF1 may serve as a scaffolding protein that regulates protein degradation in the ubiquitin-proteasome pathway.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Adenosine Triphosphatases, Amino Acid Sequence, Apoptosis, Carrier Proteins, Cell Cycle Proteins, Cell Line, Cytosol, Humans, I-kappa B Proteins, Leupeptins, Molecular Sequence Data, Proteasome Endopeptidase Complex, Protein Structure, Tertiary, Tumor Necrosis Factor-alpha, Ubiquitin
Mol. Cell. Biol.
Date: Mar. 01, 2005
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