DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases.

Cullins assemble the largest family of ubiquitin ligases by binding with ROC1 and various substrate receptors. CUL4 function is linked with many cellular processes, but its substrate-recruiting mechanism remains elusive. We identified a protein motif, the DWD box (DDB1-binding WD40 protein), and demonstrated the binding of 15 DWD proteins with ...
DDB1-CUL4A. We provide evidence supporting the critical function of the DWD box and DDB1's role as the linker mediating DWD protein association with CUL4A. A database search predicts that about one-third of WD40 proteins, 90 in humans, contain the DWD box, suggesting a potentially large number of DWD-DDB1-CUL4-ROC1 E3 ligases.
Mesh Terms:
Amino Acid Motifs, Amino Acid Sequence, Carrier Proteins, Conserved Sequence, Cullin Proteins, DNA-Binding Proteins, HeLa Cells, Humans, Molecular Sequence Data, Ubiquitin-Protein Ligases
Genes Dev.
Date: Nov. 01, 2006
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