AMSH regulates calcium-sensing receptor signaling through direct interactions.
Calcium-sensing receptor (CaR) activates intracellular pathways controlling calcium homeostasis. CaR carboxyl-terminal mutants associated with metabolic diseases suggest that unidentified proteins interact with the carboxyl-terminal region of this receptor. To address this possibility, we screened for CaR-interacting proteins using the carboxyl terminus of CaR (CaRDelta895-1075 deletion mutant). We identified AMSH, an ... ubiquitin isopeptidase, as a CaR-interacting partner. AMSH caused a decrease on the signaling properties of wild-type and mutant CaR. Our results indicate that AMSH, which has been recently characterized as a regulator of the endosomal sorting of epidermal growth factor receptor, represents a novel modulator of CaR signaling.
Mesh Terms:
Amino Acid Sequence, Cells, Cultured, Endopeptidases, Endosomal Sorting Complexes Required for Transport, Humans, Molecular Sequence Data, Peptide Fragments, Receptors, Calcium-Sensing, Signal Transduction, Ubiquitin Thiolesterase
Amino Acid Sequence, Cells, Cultured, Endopeptidases, Endosomal Sorting Complexes Required for Transport, Humans, Molecular Sequence Data, Peptide Fragments, Receptors, Calcium-Sensing, Signal Transduction, Ubiquitin Thiolesterase
Biochem. Biophys. Res. Commun.
Date: Sep. 08, 2006
PubMed ID: 16854379
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