Regulation of the anaphase-promoting complex by the COP9 signalosome.
The COP9 complex (signalosome) is a known regulator of the proteasome/ubiquitin pathway. Furthermore it regulates the activity of the cullin-RING ligase (CRL) families of ubiquitin E3-complexes. Besides the CRL family, the anaphase-promoting complex (APC/C) is a major regulator of the cell cycle. To investigate a possible connection between both complexes ... we assessed interacting partners of COP9 using an in vivo protein-protein interaction assay. Hereby, we were able to show for the first time that CSN2, a subunit of the COP9 signalosome, interacts physically with APC/C. Furthermore, we detected a functional influence of the COP9 complex regarding the stability of several targets of the APC/C. Consistent with these data we showed a genetic instability of cells overexpressing CSN2.
Mesh Terms:
Apoptosis, Cell Line, Tumor, Genomic Instability, Humans, Multiprotein Complexes, Peptide Hydrolases, Repressor Proteins, Ubiquitin-Protein Ligase Complexes
Apoptosis, Cell Line, Tumor, Genomic Instability, Humans, Multiprotein Complexes, Peptide Hydrolases, Repressor Proteins, Ubiquitin-Protein Ligase Complexes
Cell Cycle
Date: Jul. 01, 2009
PubMed ID: 19535905
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