Ubiquitin and ubiquitin-derived peptides as substrates for peptidylglycine alpha-amidating monooxygenase.

Ubiquitin (Ub) and the ubiquitin-like proteins (UBLs) mediate an array of cellular functions. These proteins contain a C-terminal glycine residue that is key to their function. Oxidative conversion of C-terminal glycine-extended prohormones to the corresponding alpha-amidated peptide is one step in the biosynthesis of bioactive peptide hormones. The enzyme catalyzing ...
this reaction is peptidylglycine alpha-amidating monooxygenase (PAM). We report herein that Ub is a PAM substrate with a (V/K)(amidation) that is similar to other known peptide substrates. This work is significant because PAM and the UBLs co-localize to the hypothalamus and the adrenal medulla and are both over-expressed in glioblastomas.
Mesh Terms:
Amino Acid Sequence, Animals, Cattle, Glycine, Glyoxylates, Mixed Function Oxygenases, Molecular Structure, Multienzyme Complexes, Oxidation-Reduction, Oxygen, Peptides, Rats, Ubiquitin
FEBS Lett.
Date: Aug. 29, 2005
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