TRAM1 participates in human cytomegalovirus US2- and US11-mediated dislocation of an endoplasmic reticulum membrane glycoprotein.
The human cytomegalovirus proteins US2 and US11 have co-opted endoplasmic reticulum (ER) quality control to facilitate the destruction of major histocompatibility complex class I heavy chains. The class I heavy chains are dislocated from the ER to the cytosol, where they are deglycosylated and subsequently degraded by the proteasome. We ... examined the role of TRAM1 (translocating chain-associated membrane protein-1) in the dislocation of class I molecules using US2- and US11-expressing cells. TRAM1 is an ER protein initially characterized for its role in processing nascent polypeptides. Co-immunoprecipitation studies demonstrated that TRAM1 can complex with the wild type US2 and US11 proteins as well as deglycosylated and polyubiquitinated class I degradation intermediates. In studies using US2- and US11-TRAM1 knockdown cells, we observed an increase in levels of class I heavy chains. Strikingly, increased levels of glycosylated heavy chains were observed in TRAM1 knockdown cells when compared with control cells in a pulse-chase experiment. In fact, US11-mediated class I dislocation was more sensitive to the lack of TRAM1 than US2. These results provide further evidence that these viral proteins may utilize distinct complexes to facilitate class I dislocation. For example, US11-mediated class I heavy chain degradation requires Derlin-1 and SEL1L, whereas signal peptide peptidase is critical for US2-induced class I destabilization. In addition, TRAM1 can complex with the dislocation factors Derlin-1 and signal peptide peptidase. Collectively, the data support a model in which TRAM1 functions as a cofactor to promote efficient US2- and US11-dependent dislocation of major histocompatibility complex class I heavy chains.
Mesh Terms:
Aspartic Acid Endopeptidases, Astrocytoma, Endoplasmic Reticulum, Glycosylation, Histocompatibility Antigens Class I, Humans, Immunoprecipitation, Membrane Glycoproteins, Membrane Proteins, Membrane Transport Proteins, Protein Transport, Proteins, RNA, Messenger, RNA, Small Interfering, RNA-Binding Proteins, Reverse Transcriptase Polymerase Chain Reaction, Subcellular Fractions, Tumor Cells, Cultured, Ubiquitin, Viral Envelope Proteins, Viral Proteins
Aspartic Acid Endopeptidases, Astrocytoma, Endoplasmic Reticulum, Glycosylation, Histocompatibility Antigens Class I, Humans, Immunoprecipitation, Membrane Glycoproteins, Membrane Proteins, Membrane Transport Proteins, Protein Transport, Proteins, RNA, Messenger, RNA, Small Interfering, RNA-Binding Proteins, Reverse Transcriptase Polymerase Chain Reaction, Subcellular Fractions, Tumor Cells, Cultured, Ubiquitin, Viral Envelope Proteins, Viral Proteins
J. Biol. Chem.
Date: Feb. 27, 2009
PubMed ID: 19121997
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