WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis.
Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B ... forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613-phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus.
Mesh Terms:
Amino Acid Sequence, Blotting, Western, CDC2 Protein Kinase, Chromatography, Gel, Cyclin B, Drosophila Proteins, Genes, Tumor Suppressor, HeLa Cells, Humans, Microscopy, Fluorescence, Mitosis, Mitotic Spindle Apparatus, Phosphorylation, Precipitin Tests, Protein Kinases, Protein-Serine-Threonine Kinases, Subcellular Fractions, Substrate Specificity
Amino Acid Sequence, Blotting, Western, CDC2 Protein Kinase, Chromatography, Gel, Cyclin B, Drosophila Proteins, Genes, Tumor Suppressor, HeLa Cells, Humans, Microscopy, Fluorescence, Mitosis, Mitotic Spindle Apparatus, Phosphorylation, Precipitin Tests, Protein Kinases, Protein-Serine-Threonine Kinases, Subcellular Fractions, Substrate Specificity
FEBS Lett.
Date: Oct. 09, 2002
PubMed ID: 12372621
View in: Pubmed Google Scholar
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