Human homologue of SETA binding protein 1 interacts with cathepsin B and participates in TNF-Induced apoptosis in ovarian cancer cells.
Lysosomal cysteine protease cathepsin B has been reported to play an important role in apoptosis of many different cancer cells, but the regulation of cathepsin B in apoptosis is poorly understood. Human homologue of SETA binding protein 1 (hSB1) was identified to interact with cathepsin B by yeast-two hybrid method, ... and the interaction was confirmed in vitro GST pull-down assay and in vivo coimmunoprecipitation experiment. hSB1 was co-localized with cathepsin B in cellular lysosomes. Our previous study has shown that TNF can induce ovarian cancer cells OV-90 apoptosis and the apoptosis process is cathepsin B-depended. Here we provide evidence that overexpression of cathepsin B-interacting protein hSB1 could suppress TNF-triggered apoptosis in OV-90 cells, but has no effect on cellular cathepsin B activity. hSB1 may function as a regulator of cathepsin B-mediated apoptosis.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Apoptosis, Cathepsin B, Female, Gene Expression, Gene Expression Profiling, Humans, Intracellular Signaling Peptides and Proteins, Lysosomes, Ovarian Neoplasms, Protein Binding, Protein Transport, Reproducibility of Results, Sequence Homology, Tumor Necrosis Factor-alpha, Two-Hybrid System Techniques
Adaptor Proteins, Signal Transducing, Apoptosis, Cathepsin B, Female, Gene Expression, Gene Expression Profiling, Humans, Intracellular Signaling Peptides and Proteins, Lysosomes, Ovarian Neoplasms, Protein Binding, Protein Transport, Reproducibility of Results, Sequence Homology, Tumor Necrosis Factor-alpha, Two-Hybrid System Techniques
Mol. Cell. Biochem.
Date: Nov. 01, 2006
PubMed ID: 16733801
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