0610009K11Rik, a testis-specific and germ cell nuclear receptor-interacting protein.

Using an in silico approach, a putative nuclear receptor-interacting protein 0610009K11Rik was identified in mouse testis. We named this gene testis-specific nuclear receptor-interacting protein-1 (Tnrip-1). Tnrip-1 was predominantly expressed in the testis of adult mouse tissues. Expression of Tnrip-1 in the testis was regulated during postnatal development, with robust expression ...
in 14-day-old or older testes. In situ hybridization analyses showed that Tnrip-1 is highly expressed in pachytene spermatocytes and spermatids. Consistent with its mRNA expression, Tnrip-1 protein was detected in adult mouse testes. Immunohistochemical studies showed that Tnrip-1 is a nuclear protein and mainly expressed in pachytene spermatocytes and round spermatids. Moreover, co-immunoprecipitation analyses showed that endogenous Tnrip-1 protein can interact with germ cell nuclear receptor (GCNF) in adult mouse testes. Our results suggest that Tnrip-1 is a testis-specific and GCNF-interacting protein which may be involved in the modulation of GCNF-mediated gene transcription in spermatogenic cells within the testis.
Mesh Terms:
Animals, Animals, Newborn, Carrier Proteins, Cell Nucleus, DNA-Binding Proteins, Gene Expression Regulation, Developmental, Male, Membrane Proteins, Mice, Mice, Inbred C57BL, Nuclear Receptor Subfamily 6, Group A, Member 1, Organ Specificity, Pachytene Stage, Protein Binding, RNA, Messenger, Receptor-Interacting Protein Serine-Threonine Kinases, Receptors, Cytoplasmic and Nuclear, Spermatids, Spermatocytes, Spermatozoa, Testis
Biochem. Biophys. Res. Commun.
Date: Feb. 22, 2008
Download Curated Data For This Publication
143394
Switch View:
  • Interactions 2